Committee Meeting II: 2D animatic

Committee members present: Prof. Michael Corrin

EDIT: Jan 19, 2011 | Crossed-off items have been incorporated into latest version of 2D animatic. The rest will be incorporated into the 3D animatic.

Segment 01, scene 01

- To give the illustration "life", redraw same image several times ("crackle")

- Also can add subtle movements to hands to further indicate pain

- Transition from gross to cellular level: consider using a radiating blur to make it more convincing

Segment 02, scene 01

- When mention "immune cells", should differentiate those cells from the other ones (highlighting, glow, slight change in hue, etc.)

- Add a list of labels for mediators (colour-coded)

- Coordinate timing between visuals and narration better: "recruit more immune cells from the blood" should come before the visuals.

Segment 02, scene 02

- May need to add a title "peripheral sensitization" to set up molecular part, sort of a part 2 to what was discussed at the cellular level

- When talking about signalling pathways, want to zoom out from close shot of the membrane with TrkA-NGF and TRPV1, to show the full signalling pathways (conveyed by arrows, etc.) to really link together what happens at the TrkA-NGF complex with the modifications to TRPV1

- Vesicle formation: To better match the orientation of the cell body shot, go from molecular level to peripheral ending first (show vesicles budding off the membrane). Then go to the cell body shot. "...affects distant sites..."

Segment 02, scene 03

- Consider adding numbers to this shot to really drive the message home - e.g. (1) transport, (2) changes in gene expression, (3) increased pronociceptive molecules

- When the ion channels are moving from the cell body to the periphery, add a zoom-in of the vesicles to show the embedded channels

- "...ultimately, greater pain sensation." - need to cut back to patient in pain...

Segment 03, scene 01

- To link together the end with the beginning, consider including the cascade at the very beginning (at least include the same background pattern in the previous shot with the patient)

Overall considerations:

- Schematic depictions/pulling back may be useful in areas explaining pathways, etc.

- Need to tighten up timing/coordination of visuals and narration (narration should precede visuals)

2D animatic

2D animatic | version 1.0

Committee Meeting: 2D animatic

Committee Members present:
Prof. Linda Wilson-Pauwels and Prof. Dave Mazierski

EDIT: Jan 19, 2011 | Crossed-off items have been incorporated into latest version of 2D animatic. The rest will be incorporated into the 3D animatic.

Comments on 2D Animatic

Title
- Change "burden" to something that relates back to pain, such as "patient's struggle", "implications on pain", etc.

Segment 01, Scene 01
- Start talk when title screen is on to move things along faster.
- Woman: Draw an older woman
- Joint: Rotate less when zooming into joint

Segment 02, Scene 01
- Make sure pain signal crosses over to other side of brain! * Crosses at level of spinal cord and goes to contralateral side --> Ask whether need to label "spinothalamic tract
- Label green particles "inflammatory mediators"
- Also list mediators along with narration
- Give blood vessel a hint of red

Segment 02, Scene 02
- Rotate membrane surface to match orientation of nociceptor (at cellular level)

Segment 02, Scene 03

Retrograde transport
- Show schematic of cell body within the dorsal root ganglion before zooming in to reinforce that the DRG contains many cell bodies
- Need to label "retrograde transport"
- Colour of NGF-TrkA - make a mixture of orange and pink particles
- Check timing before introducing "pronociceptive molecules"

Pain mediators
- "These pain mediators activate second-order neurons in the dorsal horn, firing signals to the CNS"
- The signal has to cross over to the contralateral side at the spinal cord level

Ion channels
- add "pre-assembled ion channels" to narration to clarify that the channels are manufactured in the cell body, and transported in vesicles down the axon
- Show tiny purple channels popping out of nociceptor membrane surface
- Use the term "anterograde" in narration and label "anterograde transport"

Segment 03, Scene 02
- Need to contextualize where pathway is happening by adding a membrane
- Or consider removing that part altogether, and just jump from patient with pill to pathways

- Check retrograde/anterograde transport wording