Committee Meeting: 2D Animatic (Content Advisors)

Committee Members present:
BMC supervisor: Prof. Linda Wilson-Pauwels
Content advisors: Dr. Alfredo Ribeiro-da-Silva, Ms. Geraldine Longo, Dr. Maria Osikowicz

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General
. Make text bigger for labels
. Check narration (will be fixed in final recording)
. Verified that retrograde flow is towards the cell body, and anterograde flow is away from the cell body. Should I include "anterograde" in narration or label on screen?

00:41: Cellular environment comments
. Synovial cells are mainly in the lining, so wouldn't really see.
. Include resident macrophages, not just ones elicted from the blood
. Try to embed nociceptor in the cells more (not as floating)
. Add a capillary in the scene
. Keep this simple, more general - the types of cells are difficult to differentiate.
. Give blood vessel a tint of red
. Add knobs towards the end of nociceptor (like ball and string), so not as cylindrical/bulbous at the end (actually similar to a sympathetic nerve).

01:12: No need to specify mast cell at this point, keep it more general because many cells release NGF. What should I call the cell in the narration? Narration change.

01:25: Should have resident macrophages here in addition to elicited ones.

01:45: Orientation at molecular level vs. cellular level of nociceptor/signal firing is not a big issue, but I will try rotating and show content advisors.

01:57: Clarify the signalling pathways - so PI3K is one of many pathways that occur. Narration change. (I am also considering zooming out a bit and using generic arrows to show that some sort of pathway links the phosphoryation event at TrkA with the phosphorylation of TRPV1. Would this help?)

02:07: Ion channels - need to mention in narration that TRPV1 is only one example of many ion channels located on the surface (visually, maybe show different coloured channels? Or maybe keep it simple to one). Narration change.

02:43: Appropriate to have a label with "retrograde transport"? And label with "anterograde transport" during flow of pronociceptive molecules and ion channels?

02:49: Have the background cell bodies a bit more visible, put nucleus of cell a bit more in the middle.

03:09: Make pronociceptive molecules flow both directions (can focus only on central end. Remember that mechanisms are not entirely clear, so okay to keep more general).

03:17: Draw in fusiform second order neuron in Lamina 1 (at level of C4/C5-ish) and have it cross over to contralateral side to anterior spinothalamic tract (show more of the right side of the spinal cord to accommodate). Narration change? I will label "second-order neuron", should I also label "spinothalamic tract"?

03:24: Make ion channels flow both ways (Okay to focus on the outcomes on one end only, no need to split-screen both.)

04:03: Change narration to better connect the fact that researchers are modulating increased levels of endogenous NGF (This is a subtle change and does not aim to completely sequester NGF or completely remove pain sensation, and uses pharmacological principles instead of immunological ones as in anti-NGF antibodies so don't need to worry about FDA suspension of anti-NGF clinical trials!).

04:10: Need a real pathway for this part!

Visuals needed:
- nociceptive ending showing knobs
- any resources for proteins (ion channels)
- NGF pathway for the ending

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Narration Comments (from Linda and Dave)

- Rework title - any suggestions appreciated!

Segment 02, scene 03

- "These pain mediators activate second-order neurons in the dorsal horn, firing signals to the CNS"

- add "pre-assembled ion channels" to narration to clarify that the channels are manufactured in the cell body, and transported in vesicles down the axon
- Use the terms "anterograde" in narration and label "anterograde transport"